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The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography (2016)

Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of […]
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Optimisation of polarization controlled colour tuning using nanoscale cross-shaped apertures in silver films (2016)

Nanofabrication of metamaterials based on thin metallic films have resulted in a host of different designs that support Extraordinary Optical Transmission (EOT). In contrast to the more widely studied circular-shaped apertures, cross-shaped apertures have characteristics which can be modified in response to linearly polarized light, opening up new pathways for light manipulation at the nanoscale. […]
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Dual pitch plasmonic devices for polarization enhanced colour based sensing (2016)

Plasmonic devices provide a unique sensitivity to changes in the permittivity of the immediate, near-surface environment. In this work we explore the use of dual pitch plasmonic devices combined with microfluidics for polarization enhanced colour sensing of a chemicals’ refractive index. We demonstrate that the use of cross-shaped apertures can produce polarization tunable color based […]
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Optical Ptychographic Microscopy for Quantitative Anisotropic Phase Imaging (2016)

Ptychography has recently been adapted for the recovery of the complete Jones matrix of an anisotropic specimen, using a vectorial form of the Ptychographic Iterative Engine (vPIE) for a set of linearly polarized probes. Here we show that this method can be applied to the recovery of the in-plane components of the elastic strain tensor […]
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Three-dimensional reconstruction of the size and shape of protein microcrystals using BCDI (2016)

Three-dimensional imaging of protein crystals during x-ray diffraction experiments opens up a range of possibilities for optimizing crystal quality and gaining new insights into the fundamental processes that drive radiation damage. Obtaining this information at the appropriate length-scales however is extremely challenging. One approach that has been recently demonstrated as a promising avenue for characterizing […]
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Uptake of the butyrate receptors, GPR41 and GPR43, in lipidic bicontinuous cubic phases suitable for in meso crystallization (2014)

The butyrate G-protein coupled receptors (GPCRs), GPR41 and GPR43, have been implicated in colorectal cancer and leptin production. To date their function has not been elucidated as low levels of protein expression and difficulties in producing diffraction quality crystals have hindered their structural determination. In meso crystallization, which uses an artificial lipid membrane matrix to […]
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Reconstitution of GPCRs in Lipidic Cubic Phase (LCP)

Reconstitution of GPCRs in Lipidic Cubic Phase (LCP):Comparison between human Histamine 1 and Dopamine 2Long receptor reconstitution into five different self-assembly lipids G protein-coupled receptors (GPCRs) are key pharmaceutical targets in a number of biological diseases and until the recent introduction of Lipidic Cubic Phase (LCP) crystallization, these protein structure have been extremely difficult to […]
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