Reconstitution of GPCRs in Lipidic Cubic Phase (LCP)
Reconstitution of GPCRs in Lipidic Cubic Phase (LCP):Comparison between human Histamine 1 and Dopamine 2Long receptor reconstitution into five different self-assembly lipids
G protein-coupled receptors (GPCRs) are key pharmaceutical targets in a number of biological diseases and until the recent introduction of Lipidic Cubic Phase (LCP) crystallization, these protein structure have been extremely difficult to solve. LCP crystallization has been successful in solving the structures of a number of GPCRs. However, understanding the process of LCP crystallization is limited. We have studied reconstitution of two native GPCRs, Dopamine 2 Long receptor and Histamine 1 receptor in five different self-assembly lipids; Monoolien (MO), Phytantriol (PT), Phytanoyl monoethanomide (PE), H-farnesoyl monoethanolamide (FE) and Phytanyl Diethanolamide (PDiE).